Generated:Article Title: IL2 agonists
Article Snippet: .. This cell line was generated from TF-1 cell line (ATCC CRL-2003), a human erythroleukemic cell line naturally expressing the IL2R common γ-chain, by transduction with retroviral vector encoding for the sequence of the human IL2Rβ chain (Gene ID: 3560) analogous to Farner, N. L. et al. ..
Expressing:Article Title: IL2 agonists
Article Snippet: .. This cell line was generated from TF-1 cell line (ATCC CRL-2003), a human erythroleukemic cell line naturally expressing the IL2R common γ-chain, by transduction with retroviral vector encoding for the sequence of the human IL2Rβ chain (Gene ID: 3560) analogous to Farner, N. L. et al. ..
Transduction:Article Title: IL2 agonists
Article Snippet: .. This cell line was generated from TF-1 cell line (ATCC CRL-2003), a human erythroleukemic cell line naturally expressing the IL2R common γ-chain, by transduction with retroviral vector encoding for the sequence of the human IL2Rβ chain (Gene ID: 3560) analogous to Farner, N. L. et al. ..
Retroviral:Article Title: IL2 agonists
Article Snippet: .. This cell line was generated from TF-1 cell line (ATCC CRL-2003), a human erythroleukemic cell line naturally expressing the IL2R common γ-chain, by transduction with retroviral vector encoding for the sequence of the human IL2Rβ chain (Gene ID: 3560) analogous to Farner, N. L. et al. ..
Sequencing:Article Title: IL2 agonists
Article Snippet: .. This cell line was generated from TF-1 cell line (ATCC CRL-2003), a human erythroleukemic cell line naturally expressing the IL2R common γ-chain, by transduction with retroviral vector encoding for the sequence of the human IL2Rβ chain (Gene ID: 3560) analogous to Farner, N. L. et al. ..
Activity Assay:Article Title: Bispecific antibody that binds to CD116 and CD131
Article Snippet: .. In the activity analysis, a TF-1 cell line (ATCC, CRL-2003) exhibiting a proliferation activity depending on GM-CSF signals was used. .. TF-1 cells, which were subcultured and maintained in an RPMI 1640 medium (nacalai tesque) supplemented with 2 ng/mL recombinant human GM-CSF (R&D Systems), 10% FBS, and 50 μg/mL Gentamicin Sulfate Solution, were centrifuged, washed 3 times with an Macrophage-SFM medium (GIBCO), suspended in an Macrophage-SFM medium to 2.5×105 cells/mL, and dispensed into a 96-well plate (Greiner) at 80 μL per well and 2.0×104 cells/well.
In Vitro:Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering.
Article Snippet: .. In Vitro Functional Assay Using Cell Culture The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 ◦C and 5% carbon dioxide.
Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering
Article Snippet: .. The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003 TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 °C and 5% carbon dioxide.
Functional Assay:Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering.
Article Snippet: .. In Vitro Functional Assay Using Cell Culture The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 ◦C and 5% carbon dioxide.
Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering
Article Snippet: .. The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003 TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 °C and 5% carbon dioxide.
Cell Culture:Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering.
Article Snippet: .. In Vitro Functional Assay Using Cell Culture The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 ◦C and 5% carbon dioxide.
Derivative Assay:Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering.
Article Snippet: .. In Vitro Functional Assay Using Cell Culture The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 ◦C and 5% carbon dioxide.
Article Title: Satisfying QTPP of Erythropoietin Biosimilar by QbD through DoE-Derived Downstream Process Engineering
Article Snippet: .. The in vitro functional assay was performed using the TF-1 cell line (ATCC, Manassas, VA, USA; ATCC number: CRL-2003 TM and lot number: 64161542), which was originally derived from a patient diagnosed with erythroleukemia. .. TF-1 cells were maintained in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific, USA), 1% PS (Thermo Fisher Scientific, USA), and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 °C and 5% carbon dioxide.
Recombinant:
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ATCC is a verified supplier 
ATCC manufactures this product 